Table 3.

Estimates of observed richness and correlation with original sequencing depth for data processed with DADA2. Estimates shown are calculated on data processed without use of a prior.

Study	Processing	Sub-sampling	$\rho$1	Mean observed richness (SD)	Mean singletons
Aho et al., 2019 (C)	Unpooled	None	0.54	256.12 (70.30)	1.00
		Post	0.50	241.65 (63.70)	17.99
		Pre	0.07	101.80 (17.80)	0.43
	Pooled	None	0.23	820.02 (217.00)	251.00
		Post	−0.02	469.16 (110.00)	145.98
		Pre	−0.01	388.27 (80.70)	125.14
Almeida-Santos et al., 2021 (A)	Unpooled	None	0.34	372.90 (127)	10.80
		Post	0.27	309.76 (94.7)	45.39
		Pre	0.04	83.90 (14.1)	1.32
	Pooled	None	0.08	3178.07 (1104)	2044.85
		Post	−0.40	971.76 (352)	554.71
		Pre	−0.01	704.66 (150)	407.93
Manor et al., 2018 (B)	Unpooled	None	0.42	226.53 (92.20)	0.00
		Post	0.36	216.05 (84.90)	12.33
		Pre	0.27	106.53 (43.50)	0.00
	Pooled	None	0.30	592.53 (166.00)	129.40
		Post	0.09	384.10 (110.00)	94.82
		Pre	0.15	296.28 (77.60)	66.93