Table 1. Effect of diazepam on ischemia-reperfusion injury (IRI)-induced alterations in relative and absolute heart weight, serum creatine kinase-MB, lactate dehydrogenase, alanine aminotransferase in rats.
| Parameters | Sham | IRI Control | IRI + Dil (10) | IRI + Dia (1) | IRI + Dia (2.5) | IRI + Dia (5) | Dia (5) |
|---|---|---|---|---|---|---|---|
| Heart weight (g) | 0.40 ± 0.01 | 0.81 ± 0.03# | 0.48 ± 0.03*,$ | 0.75 ± 0.07 | 0.63 ± 0.07*,$ | 0.54 ± 0.02*,$ | 0.50 ± 0.03 |
| Heart weigh/ Body weight (×10−3) | 1.71 ± 0.04 | 3.52 ± 0.15# | 2.14 ± 0.11*,$ | 3.25 ± 0.28 | 2.76 ± 0.30*,$ | 2.30 ± 0.09*,$ | 2.17 ± 0.13 |
| Serum CK-MB (IU/I) | 1,073.00 ± 44.11 | 2,062.00 ± 56.63# | 1,185.00 ± 39.01*,$ | 2,048.00 ± 64.43 | 1,632.00 ± 51.22*,$ | 1,435.00 ± 55.70*,$ | 1,033.00 ± 56.47 |
| Serum LDH (IU/I) | 1,212.00 ± 73.23 | 2,634.00 ± 107.50# | 1,627.00 ± 92.86*,$ | 2,685.00 ± 111.10 | 2,014.00 ± 95.56*,$ | 1,638.00 ± 87.84*,$ | 1,222.00 ± 68.46 |
| AST (mg %) | 110.90 ± 11.89 | 358.70 ± 11.35# | 139.00 ± 9.26*,$ | 334.80 ± 10.70 | 249.50 ± 8.68*,$ | 139.60 ± 11.77*,$ | 121.00 ± 8.98 |
Data are expressed as mean ± S.E.M (n=6) and analyzed by one-way ANOVA followed by Tukey’s multiple range tests. *P<0.05 as compared to the IRI-control group, #P<0.05 as compared to the sham, $P<0.05 as compared to one another. IRI: Ischemia-reperfusion Injury control rats; Dil (10): diltiazem (10 mg/kg, p.o.) treated rats; Dia (1): diazepam (1 mg/kg, p.o.); Dia (2.5): diazepam (2.5 mg/kg, p.o.) and Dia (5): diazepam (5 mg/kg, p.o.) treated rats. AST: alanine aminotransferase; CK-MB: creatine kinase-MB; LDH: lactate dehydrogenase.