Table 2. Effect of diazepam on ischemia-reperfusion injury (IRI)-induced alterations electrocardiographic, hemodynamic, and left ventricular function tests changes in rats.
| Parameters | Sham | IRI Control | IRI + Dil (10) | IRI + Dia (1) | IRI + Dia (2.5) | IRI + Dia (5) | Dia (5) |
|---|---|---|---|---|---|---|---|
| Heart Rate (BPM) | 369.20 ± 10.94 | 271.00 ± 10.89# | 349.80 ± 11.42*,$ | 287.20 ± 7.51 | 319.00 ± 9.01*,$ | 343.00 ± 11.04*,$ | 351.00 ± 8.80 |
| QRS interval (msec) | 12.80 ± 0.58 | 32.40 ± 0.68# | 17.40 ± 0.68*,$ | 28.80 ± 0.37 | 22.60 ± 0.75*,$ | 20.60 ± 0.93*,$ | 13.60 ± 0.51 |
| QT Interval (msec) | 48.17 ± 2.59 | 88.50 ± 1.57# | 57.50 ± 2.01*,$ | 86.00 ± 3.14 | 72.83 ± 2.55*,$ | 64.17 ± 2.06*,$ | 57.50 ± 2.34 |
| QTc Interval (msec) | 125.30 ± 5.30 | 175.70 ± 5.89# | 146.80 ± 3.34*,$ | 169.80 ± 4.55 | 154.20 ± 6.69*,$ | 144.20 ± 6.81*,$ | 135.50 ± 5.92 |
| RR interval (msec) | 144.30 ± 6.09 | 204.50 ± 4.64# | 161.80 ± 3.44*,$ | 198.80 ± 3.37 | 183.70 ± 5.59*,$ | 170.80 ± 5.16*,$ | 152.50 ± 3.73 |
| SBP (mmHg) | 106.70 ± 1.87 | 161.30 ± 4.72# | 124.00 ± 4.48*,$ | 151.80 ± 4.09 | 137.30 ± 5.06*,$ | 119.30 ± 1.82*,$ | 108.70 ± 2.89 |
| DBP (mmHg) | 83.50 ± 3.72 | 117.50 ± 2.51# | 89.33 ± 3.70*,$ | 110.50 ± 2.79 | 98.50 ± 3.22*,$ | 96.83 ± 4.11*,$ | 91.00 ± 4.05 |
| LVEDP (mmHg) | 5.33 ± 0.21 | 11.67 ± 0.56# | 7.50 ± 0.43*,$ | 11.17 ± 0.75 | 8.67 ± 0.67*,$ | 7.50 ± 0.56*,$ | 5.83 ± 0.70 |
| Maxdp/dt | 4,011.00 ± 151.70 | 1,995.00 ± 134.50# | 3,656.00 ± 117.60*,$ | 2,518.00 ± 124.10 | 2,858.00 ± 126.40*,$ | 3,499.00 ± 116.90*,$ | 3,880.00 ± 167.80 |
| Mindp/dt | −2,708.00 ± 88.37 | −1,970.00 ± 74.60# | −2,382.00 ± 69.35*,$ | −1,886.00 ± 36.34 | −2,245.00 ± 58.68*,$ | −2,547.00 ± 35.78*,$ | −2,530.00 ± 85.90 |
| Pressure time index | 17.33 ± 0.33 | 24.33 ± 0.33# | 20.17 ± 0.75*,$ | 23.67 ± 0.95 | 21.33 ± 0.67*,$ | 21.83 ± 0.40*,$ | 18.83 ± 0.87 |
| Contractility index | 56.33 ± 1.50 | 33.83 ± 1.17 | 47.17 ± 1.70*,$ | 35.00 ± 1.93 | 42.17 ± 1.40*,$ | 44.17 ± 1.78*,$ | 56.50 ± 1.09 |
| Tau (msec) | 3.50 ± 0.56 | 10.83 ± 0.65 | 5.83 ± 0.54 | 10.17 ± 0.48 | 9.17 ± 0.31*,$ | 6.50 ± 0.67*,$ | 5.00 ± 0.73 |
Data are expressed as mean ± S.E.M (n=6) and analyzed by one-way ANOVA followed by Tukey’s multiple range tests. *P<0.05 as compared to the IRI-control group, #P<0.05 as compared to the sham, $P<0.05 as compared to one another. IRI: ischemia-reperfusion injury control rats; Dil (10): diltiazem (10 mg/kg, p.o.) treated rats; Dia (1): diazepam (1 mg/kg, p.o.); Dia (2.5): diazepam (2.5 mg/kg, p.o.) and Dia (5): diazepam (5 mg/kg, p.o.) treated rats. SBP: systolic blood pressure; DBP: diastolic blood pressure; LVEDP: left ventricular end-diastolic pressure.