Table 3. Effect of diazepam on ischemia-reperfusion injury (IRI)-induced alterations cardiac oxido-nitrosative stress and ATPase enzymes in rats.
| Parameters | Sham | IRI Control | IRI + Dil (10) | IRI + Dia (1) | IRI + Dia (2.5) | IRI + Dia (5) | Dia (5) |
|---|---|---|---|---|---|---|---|
| SOD (U/mg of protein) | 10.53 ± 0.27 | 3.73 ± 0.44# | 9.23 ± 0.48*,$ | 4.13 ± 0.17 | 6.61 ± 0.35*,$ | 8.71 ± 0.46*,$ | 10.48 ± 0.65 |
| GSH (µg/mg protein) | 32.94 ± 1.01 | 17.78 ± 1.27# | 29.13 ± 0.98*,$ | 18.85 ± 1.26 | 22.26 ± 0.85*,$ | 25.80 ± 1.03*,$ | 30.61 ± 1.10 |
| MDA (nmol/l/mg of protein) | 2.52 ± 0.21 | 5.69 ± 0.29# | 3.33 ± 0.30*,$ | 5.04 ± 0.23 | 4.81 ± 0.27*,$ | 3.97 ± 0.23*,$ | 3.11 ± 0.19 |
| NO (µg/mg of protein) | 216.40 ± 30.70 | 704.00 ± 28.39# | 317.40 ± 37.31*,$ | 635.20 ± 27.03 | 516.00 ± 29.32*,$ | 377.40 ± 37.64*,$ | 247.40 ± 24.21 |
| Na+K+ATPase (µmol/mg of protein) | 5.78 ± 0.31 | 2.86 ± 0.37# | 4.99 ± 0.38*,$ | 3.09 ± 0.18 | 4.54 ± 0.18*,$ | 5.01 ± 0.39*,$ | 5.17 ± 0.33 |
| Ca2+ATPase (µmol/mg of protein) | 3.44 ± 0.33 | 1.61 ± 0.31# | 3.14 ± 0.21*,$ | 1.61 ± 0.25 | 2.33 ± 0.27*,$ | 2.86 ± 0.36*,$ | 3.01 ± 0.25 |
Data are expressed as mean ± S.E.M (n=6) and analyzed by one-way ANOVA followed by Tukey’s multiple range tests. *P<0.05 as compared to the IRI-control group, #P<0.05 as compared to the sham, $P<0.05 as compared to one another. IRI: ischemia-reperfusion Injury control rats; Dil (10): diltiazem (10 mg/kg, p.o.) treated rats; Dia (1): diazepam (1 mg/kg, p.o.); Dia (2.5): diazepam (2.5 mg/kg, p.o.) and Dia (5): diazepam (5 mg/kg, p.o.) treated rats. SOD: superoxide dismutase; GSH: glutathione peroxidase; MDA: malondialdehyde; NO: nitric oxide.