(A) Morphological changes of tibialis anterior (TA) muscles in the
Intact/Cardiotoxin (CTX) group compared with those in the ovariectomized (OVX)/CTX
group at days 3, 7, 10 and 14 (D3, D7, D10 and D14) after CTX injection. Arrows
indicate cell infiltration. Asterisks indicate myofiber degeneration. Arrowheads
indicate newly formed myotubes, scale bar: 50 µm. (B) Comparison of the
diameters of regenerated myotubes at different time points after CTX injection
between the Intact/CTX group and OVX/CTX group. The boxes represent the distribution
of diameters between first quartile (Q1) and third quartile (Q3). The horizontal
line between Q1 and Q3 represents the median (Q2) of regenerated myotubes in each
group. Outliers are not shown in the graph. ×indicates average diameter of
regenerated myotubes in each group, ** indicates significant difference between
groups, # indicates significant difference from intact groups (non-injured), †
indicates significant difference from OVX groups (non-injured),
P<0.01. ND, not detected.