Figure 2.
Comparison of the properties of PTM targets in E. coli
(Abbreviation: Enzyme targets of transcription (Tr), posttranscription (Pr), acetylation (Ac), phosphorylation (Ph), unknown regulation (Un))
(A) Enzymes that impact growth when knocked out are highly likely to be acetylated. The ANOVA p value comparing the differences in means is shown in the title of the boxplot.
(B) Enzymes catalyzing reactions with high maximum flux are likely to be targets of phosphorylation. The ANOVA p value comparing the differences in means is shown in the title of the boxplot.
(C) Enzymes with high molecular weight are likely to be targets of phosphorylation. Similar to yeast, reaction essentiality (A), maximum flux (B), and molecular weight (C) are predictive of regulation by acetylation and phosphorylation (Vmax, MW) respectively during transition to stationary phase. Analysis of condition-specific PTM targets across 11 growth conditions.
(D–F) Proteins that were found to be conditionally essential (growth < wild type glucose) based on FBA (D) or Transposon sequencing (Z score < −2) (E) were more likely to be acetylated (p value = 0.02 & 0.0011 for FBA and Tn-seq respectively). (F) Enzymes that are predicted to have high maximal flux (Vmax > 90th percentile) in a condition were likely to be phosphorylated compared to those with low maximal flux (p value = 0.008).