. 2021 Dec 9;101(3):101637. doi: 10.1016/j.psj.2021.101637

Table 1.

Effect of different levels of gamma-oryzanol and gamma-oryzanol nanoparticles on motility parameters of rooster sperm assessed by CASA after freeze-thawing.

	Treatments
Parameters	C	GO20	GO40	GO60	GO80	GO100	CN	GO20N	GON40	GON60	GON80	GON100	SEM
TM (%)	37.3^e	40.9^de	43.5^de	52.4^c	60.2^b	39.5^e	38.6^e	43.2^de	47.8^cd	66.3^ab	71.7^a	41.8^de	1.59
PM (%)	16.9^d	17.3^cd	18.2^cd	18.5^cd	22.4^bc	17.2^cd	17.0^d	18.1^cd	19.1^cd	26.8^b	34.9^a	17.3^cd	1.14
VAP (µm/s)	30.5^c	30.7^c	31.1^c	31.8^c	33.1^bc	30.3^c	30.1^c	30.9^c	32.6^bc	36.3^ab	38.2^a	30.1^c	0.88
VSL (µm/s)	16.8^c	17^bc	17.4^bc	18.3^bc	19.1^abc	16.9^c	16.9^c	17.3^bc	18.6^bc	20.9^ab	22.8^a	16.9^c	0.83
VCL (µm/s)	54.2^b	54.4^b	55.2^ab	56.1^ab	57.6^ab	54.3^b	54.3^b	54.8^b	56.9^ab	57.5^ab	60.3^a	54.8^b	1.14
LIN (%)	30.9	30.9	30.9	30.9	30.9	30.9	30.9	30.9	30.9	30.9	30.9	30.9	1.47
STR (%)	55.4	56.1	56.3	57.7	58	56	56.5	56.4	57.1	58.2	59.6	56.1	3.1
BCF (Hz)	16.2	16.4	16.7	16.9	17.1	16.4	16.3	16.8	17	17.7	18.4	16.5	0.94
ALH (µm)	5.5	5.3	5.1	5	4.6	5.2	5.4	5.1	4.8	4.5	4.3	5.1	0.31

Abbreviations: LIN, linearity; PM, progressive motility; TM, total motility; VAP, average path velocity; VCL, curvilinear velocity; VSL, straight-line velocity.

Lake extender including gamma-oryzanol (GO) at 0 (C) (control group), 20 (GO20), 40 (GO40), 60 (GO60), 80 (GO80), and 100 (GO100) µM and gamma-oryzanol nanoparticles (GON) at 0 (CN), 20 (GON20), 40 (GON40), 60 (GO60), 80 (GON80), and 100 (GON100) µM.

Different superscripts within the same row indicate significant differences among groups (P < 0.05).