Figure 3.

Evaluation of sunscreen formulations on the morphology and number of fibroblasts of reconstructed skins exposed to UVA1. At 48 hours after UVA1 exposure, sections of reconstructed skins were realized to perform (a) histology, (b) vimentin immunostaining, and (c) fibroblasts counting. Four independent experiments were performed. In panel a, circles and arrows indicate epidermal alterations and depth of fibroblasts disappearance, respectively. In panel b, vimentin staining appears green and is located in fibroblasts’ cytoplasm. Cell nuclei counterstained using propidium iodide appear in red. In panel c, each point shows the mean value of fibroblasts number per field ± SEM. Asterisk (∗) indicates a significant difference from the Reference vitro; degree symbol (°) indicates significant difference from Formula vitro 0.7% MCE (Student’s t-test, P < 0.05). Fla, formula; MCE, Methoxypropylamino Cyclohexenylidene Ethoxyethylcyanoacetate; Ref, reference.