Table 4.

Evaluation of Sunscreen Formulations on the Levels of Secreted Proteins in Culture Medium of Reconstructed Skins Exposed to UVA1

Amount of Protein in the Condition 0 J/cm2, No Formula		MMP1 18.3 ± 4.6 ng/ml	IL-1RA 23.0 ± 5.6 ng/ml	IL-6 0.7 ± 0.3 μg/ml	GM-CSF 54.4 ± 13,6 ng/ml	IL-8 2,1 ± 1,2 μg/ml
0 J/cm2	No formula	1 ± 0.2	1 ± 0.1	1 ± 0.1	1 ± 0.1	1 ± 0.2
40 J/cm2		2.7 ± 0.9	7.1 ± 2.1	2.4 ± 1.0	3.3 ± 1.2	2.7 ± 1.4
40 J/cm2	Ref vitro	1.2 ± 0.9	6.8 ± 2.1	1.2 ± 1.0	2.4 ± 1.2	1.4 ± 1.4
	Fla vitro 0.7% MCE	1.3 ± 0.3	5.7 ± 1.2	1.4 ± 0.3	2.5 ± 1.1	1.5 ± 0.8
	Fla vitro 1.5% MCE	1.1 ± 0.3	4.0 ± 0.81	1.4 ± 0.6	1.5 ± 0.5	2.3 ± 1.5
60 J/cm2	Ref vitro	5.6 ± 3.8	33.1 ± 13.0	4.6 ± 3.3	10.9 ± 10.1	5.9 ± 4.7
	Fla vitro 0.7% MCE	1.4 ± 0.51	7.2 ± 2.21	1.3 ± 0.41	2.4 ± 1.11	1.9 ± 0.62
	Fla vitro 1.5% MCE	1.9 ± 0.72	7.4 ± 1.41	2.1 ± 1.0	2.2 ± 0.51	3.1 ± 1.0
80 J/cm2	Ref vitro	11.1 ± 7.6	76.5 ± 27.6	4.9 ± 2.2	14.2 ± 7.9	6.2 ± 2.3
	Fla vitro 0.7% MCE	3,8 ± 3.11	23.1 ± 18.42	1.9 ± 1.31	6.8 ± 6.52	3.1 ± 1.71
	Fla vitro 1.5% MCE	2.2 ± 1.01	12.6 ± 3.51	1.4 ±- 0.51	3.5 ± 2.31	2.2 ± 0.81

Abbreviations: CI, confidence interval; Fla, formula; MCE, Methoxypropylamino Cyclohexenylidene Ethoxyethylcyanoacetate; MMP1, matrix metalloproteinase 1; Ref, reference.

The amount of secreted proteins was measured 48 hours after UVA1 exposure by ELISA in culture media of reconstructed skins protected or not by a Fla. This was divided by the average number of living fibroblasts. Values of control samples (no UV, no formula) are indicated in the first line and were then adjusted to the 1 value. Values are expressed as means of four independent experiments ± 95% CI. The significant difference between 40 J/cm² and 0 J/cm² in the absence of formula is reported in bold (P < 0.05).

¹Significant difference from reference vitro (P < 0.05).

²Significant difference from reference vitro (0.05 < P < 0.1) in Student’s t-test.