Figure 6.

In vivo antimetastatic effects of S@C-PLGA NPs. Histopathological analysis and quantification of metastatic foci for lung, liver, and lymph node of untreated control mice (A) and 4T1 tumor-bearing mice treated with multiple doses of saline (B), Stattic (24 mg/kg) (C) and S@C-PLGA NPs (24 mg Stattic eqv./kg) (D). No metastatic foci were observed in heart, kidney and spleen. Quantification of number of metastatic foci found in lung (E) and liver (F) by observing 5 and 8 images in lung and liver, respectively, for each mouse in all the treatment groups. Average sizes of metastatic foci found in lung (G) and liver (H) for each treatment group. Data represent the mean number of metastatic foci ± SEM (n=4); *, **, *** and ****Indicate p <0.05, 0.01, 0.001 and 0.0001, respectively as assessed by One-way ANOVA with Dunnett’s post-hoc test. Yellow arrows indicate metastatic foci.