Figure EV5. Biophysical and biochemical properties of PDIA3^C57Y .

1. Circular dichroism (CD) analysis of recombinant wild‐type PDIA3 and PDIA3^C57Y. Average traces of CD spectroscopic scans are shown.
2. Purified recombinant wild‐type PDIA3 and PDIA3^C57Y were treated with proteinase K and analyzed by SDS–PAGE with Coomassie blue staining as shown in Fig 6H. n = 4 independent reactions. Bar graph shows quantification of protein digestion. Data are shown as mean ± s.e.m. and statistical analysis performed using two‐tailed Student's t‐test.
3. Dynamic light scattering determination of mean molecular weight (MW) and percent polydispersity of wild‐type PDIA3 and PDIA3^C57Y. Data are shown as mean ± s.d.

Source data are available online for this figure.