Table 1.
Summary of peptides used in the cellulose peptide array.
| ID | Sequence | UniProt ID | Protein Name | Membrane hits | Lag phase | Amplitude | Fibril Length (TEM) | Dye binding | Fibril morphology (AFM) | Biosensor cells | Amyloid plaques (Xiong et al(2019a, b) | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P1 | HRKSVFFVGQL | Q562E7‐1 | WD repeat‐containing protein 81 | Yes | *** ↑ | ns | ns | ns | nt | nt | Yes | Endolysosomal, involved in aggrephagy |
| P2 | NPKLDFFKNFL | O94822 | E3 ubiquitin‐protein ligase listerin | Yes | **** ↓ | * ↓ | ns | ns | nt | nt | Yes | Part of ribosomal quality control complex |
| P3 | AVLRFFNEVFK | P23786 | Carnitine O‐palmitoyltransferase 2 | Yes | **** ↓ | ns | **** ↑ | ns | x | ****↑ | Yes | Mitochondrial |
| P4 | SQRLVGFALRR | P51790‐2 | H(+)/Cl(‐) exchange transporter 3 | Yes | **** ↑ | ns | ns | ns | nt | *↑ | Yes | Endolysosomal, involved in acidification |
| P5 | FMMTVFFAKKL | O95196‐1 | Chondroitin sulphate proteoglycan 5 | Yes | **** ↑ | **** ↓ | ** ↑ | * (curcumin) | x | ns | Neurogenesis, trans‐synaptic signalling | |
| P6 | KHKPVAFAVRT | P54284‐1 | Voltage‐dependent L‐type calcium channel subunit beta‐3 | Yes | ns | ns | ns | ns | nt | ns | Yes | Presynaptic depolarization and calcium channel opening |
| P7 | KGTVFFDEFTF | O95497 | Pantetheinase | Yes | ns | ns | ** ↑ | ns | nt | ns | Inflammatory response | |
| P8 | LPLVIFHELTK | O15354 | Prosaposin receptor GPR37 | Yes | *** ↓ | **** ↓ | ** ↑ | ns | x | nt | Receptor for neuroprotective factor | |
| P9 | DKLQFFEERRR | Q15772‐5 | Striated muscle preferentially expressed protein kinase | No | ns | ns | ns | ns | nt | nt | Possible growth regulator in muscle | |
| P10 | PLSRVFFASWR | P05164‐1 | Myeloperoxidase | Yes | *** ↑ | ns | ns | ns | nt | nt | Response to oxidative stress | |
| P11 | DFRVFFQELVE | Q92985‐2 | Interferon regulatory factor 7 | Yes | *** ↓ | ns | ns | ns | nt | nt | Transcription factor regulating inflammation | |
| P12 | QRLVGFALRRD | Q6ZNZ2 | cDNA FLJ26854 | Yes | *** ↑ | ns | **** ↓ | ns | x | nt | unknown | |
| P13 | SGLSLFAETIW | O00322 | Uroplakin‐1a | Yes | ns | ns | ns | ns | nt | nt | Component of asymmetric unit membrane | |
| P14 | SNLQFKAERIK | Q8TDM6‐1 | Disks large homolog 5 | Yes | ns | ns | ** ↓ | ns | nt | nt | Hippo regulator, synaptogenesis | |
| P15 | LLAVFFALGLE | Q08462 | Adenylate cyclase type 2 | No | ns | *** ↑ | ns | ns | nt | nt | Yes | Catalyses cAMP in response to G‐protein signalling |
| P16 | LRKLVRGATLD | O95273‐1 | Cyclin‐D1‐binding protein 1 | Yes | ns | ns | **** ↓ | ** (pFTAA) | nt | nt | Negative regulator of cell cycle progression | |
| P17 | PRKLDFFRSEK | O15031 | Plexin‐B2 | Yes | ns | ns | ns | ** (pFTAA) | nt | nt | Yes | Cell surface receptor, synapse assembly |
| P18 | FYLFFFTEKIL | Q15043‐1 | Metal cation symporter ZIP14 | Yes | ** ↓ | ns | ns | ns | nt | nt | Yes | Metal ion membrane transporter |
| P19 | FIFLRFFAPAI | Q14644 | Ras GTPase‐activating protein 3 | Yes | ** ↓ | ns | **** ↓ | ns | nt | nt | Yes | Inhibitory regulator of the Ras‐cyclic AMP pathway |
| P20 | YNNLVSFASPL | Q96Q15‐1 | Serine/threonine‐protein kinase SMG1 | No | ** ↓ | ns | **** ↓ | ns | nt | nt | Genotoxic stress response | |
| P21 | SRRLVPFAQFI | Q71RG8 | FP2025 | Yes | ** ↓ | ns | **** ↓ | ns | nt | nt | Unknown | |
| P22 | ADKLVFFVNGR | P47989 | Xanthine dehydrogenase/oxidase | Yes | ** ↓ | ns | ns | ** (pFTAA) | nt | nt | Contributes to the generation of reactive oxygen species | |
| P23 | APQLVFAARAV | Q9BZ82 | FKSG39 | No | ** ↓ | ns | ns | *** (pFTAA) | nt | nt | Unknown | |
| P24 | VKTLVFFFLES | B1AMT5 | Cohesin subunit SA‐2 | No | * ↓ | ns | ns | ns | nt | nt | DNA replication | |
| P25 | IPKLVNFATLG | P08922 | Proto‐oncogene tyrosine‐protein kinase ROS | Yes | **** ↓ | * ↓ | ns | ns | nt | nt | Orphan receptor tyrosine kinase (RTK) | |
| P26 | SKLLVFFRTEA | A2RRP1‐1;A2RRP1‐2 | Neuroblastoma‐amplified sequence | Yes | ns | **** ↓ | ns | * (curcumin) | nt | nt | Involved in Golgi‐to‐endoplasmic reticulum (ER) retrograde transport | |
| P27 | VGLLVQFAFRE | Q99698‐1 | Lysosomal‐trafficking regulator | Yes | **** ↑ | ns | ns | ns | nt | nt | Regulates and/or fission of intracellular vesicles such as lysosomes | |
| P28 | PVQLVNFAYRD | Q2M3C6‐1;Q2M3C6‐2 | Transmembrane protein 266 | No | ns | ns | ns | ns | nt | ns | Post‐synaptic voltage‐sensor protein | |
| P29 | IFSLVFTAVER | Q99463;B4DRU5 | Putative neuropeptide Y receptor type 6 | No | ns | ns | **** ↑ | ns | nt | nt | Ligand unknown | |
| P30 | GYVLVFDAWTK | P51828 | Adenylate cyclase type 7 | Yes | ns | *** ↓ | ns | ns | nt | nt | Catalyses cAMP in response to G‐protein signalling | |
| P31 | DWRLVFGAKEI | P10323 | Acrosin | Yes | ns | ns | ns | ns | nt | nt | Major protease of mammalian spermatozoa | |
| P32 | LALLVFFGDVG | Q5GH73‐1;Q96KT3 | XK‐related protein 6 | No | **** ↑ | ns | ns | ns | nt | nt | Integral membrane protein, enriched in brain |
The ID is an arbitrary number assigned to each peptide, the full sequence is shown. The UniProt ID (UniProt Consortium, 2008) of the protein from which the segment was taken is provided, as well as its name. Then a summary is provided of its results in the various assays: binding on the peptide array, lag phase and amplitude in the Aβ aggregation kinetics assay using ThT, fibril length determined by TEM, fluorescence emission spectra of amyloid sensor dyes, fibril morphology by AFM and Aβ aggregation in the sensor cell line. Finally, the presence of the protein in patient plaque‐derived data set from Xiong et al (2019a, b) is shown, and finally some comments on function and subcellular localization. * indicates significance (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001), ↑ increase compared to Aβ, ↓ decreased compared to Aβ, ns: no significance, nt: not tested, x: observed difference