Figure 3.

Single quadrupole enhanced in-source multiple fragment ion monitoring and triple quadrupole MRM limit of quantitation (LOQ) and dynamic range. Chemical names (a) and structures (b), dynamic ranges (c, d), LC chromatograms at LOQ levels in solvent (e), and LC chromatograms in bacterial cell extracts (f) of four representative molecules with different physicochemical properties. Dynamic range data and LC chromatograms acquired in both single and triple quadrupoles are shown; the linearity was calculated based on the log10 transformed values; the ion used with enhanced in-source fragmentation multiple fragment ion monitoring and the transition used in MRM are shown next to the linear curve. Valine and glucose-6-phosphate were acquired using HILIC chromatography; C20 sphingosine and eicosapentaenoic acid were acquired using reverse phase chromatography.