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. 2021 Dec 17;13(1):134–139. doi: 10.1021/acsmedchemlett.1c00629

Figure 4.

Figure 4

Inhibition of the estrogen-dependent transcriptional activity of ERα by LCL-ER(dec). MCF-7 cells were transiently transfected with a luciferase reporter plasmid containing three tandem copies of the ERE and control Renilla luciferase plasmid-SV40. After 24 h, the cells were further transfected with 10 μM concentrations of the indicated decoys in the presence of 3 nM β-estradiol for 24 h. The ERα-dependent transcriptional activity was evaluated by a luciferase assay, and the relative luciferase activity was normalized by designating the activity of the nontreated control (column 1) as 100%. The data represent the mean ± SD (n = 4). P values were determined using the unpaired two-tailed Student’s t-test. N.S., not significant; ** P < 0.005; *** P < 0.001.