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. 2022 Jan 17;24:24. doi: 10.1186/s13075-021-02693-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Early inflammatory infiltrates at entheses show abundant neutrophils. A Clinical peripheral inflammation scores recorded weekly over a period of 11 weeks post intraperitoneal curdlan injection (n = 6 SKG, n = 4 BALB/c controls). B Prominent inflammatory infiltrates (red arrowheads) in female SKG mice (6 weeks post curdlan injection) are seen in H&E-stained sections (×4 magnification) at the spine base (SB), a region of high mobility. Lesser infiltrates are present in other regions of the spine, including the thoracolumbar (TL), lumbosacral (LS) and mid tail (MT) sites. C, D Immunohistochemistry (IHC) demonstrating abundant neutrophils, as stained by anti-MPO antibodies, present in SKG spine base enthesis (C) and at ankle enthesis (D). Black arrow: vertebral body. Red arrowhead: intervertebral disc. Black arrowhead: site of insertion of a tendon. Characteristic neutrophil trilobed nuclei can be seen in enlarged insets (bottom right)