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. 2022 Jan 17;41:26. doi: 10.1186/s13046-022-02245-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Knocking down DLG1 promoted cell migration, invasion, and EMT. A Quantitative PCR analysis of DLG1 knockdown efficiency in GH3, MCF-7, and 293 T cells. Results are expressed as mean ± SD; P values by one-way ANOVA compared with control (si-NC). B DLG1 knockdown significantly increased N-cadherin, Vimentin, and Snail expression but decreased E-cadherin expression in GH3, MCF-7, and 293 T cells. C Scratch assay of 293 T and MCF7 cells with or without DLG1 siRNA; scale bar: 200 μm. Relative scratch width over time shown below (n = 3; mean ± SD shown below; P values by two-way ANOVA). D Transwell assay of 293 T cells transfected with negative control (si-NC) and si-DLG1; scale bar: 200 μm. Data are presented as the mean ± SD from three independent experiments. P values by one-way ANOVA; **P < 0.01; ***P < 0.001; ****P < 0.0001, relative to control (si-NC). E Confocal immunofluorescence images in wound healing migration assays of MCF7 cells. After transfection with siRNAs for 48 h, MCF7 cells were scratched to allow cell migration. Cells were fixed after migrating for 12 h, and then stained with phalloidin (green) and DAPI (blue) to reveal F actin and nuclei, respectively; scale bar: 50 μm