FIG. 5.

Results of PCR amplification for the babesial nuclear small-subunit rRNA gene with template DNAs prepared from archived blood samples of the eight donors (lanes 1 to 8, respectively) and from the patient's blood (lane 9). The amplification product after the first-round PCR with primers Bab 1 and Bab 4 and those after the nested PCR with primers Bab 2 and Bab 3 were analyzed for the patient's and the donor's DNA samples, respectively. φ×174/Hae III DNA was used as a DNA size maker (lanes M).