Figure 6.

Single-cell trajectories of melanoma resistance to mitogen-activated protein kinase inhibitor treatment. Single-cell RNA-seq data were generated from 4 different groups of A375 cells (S, RV, RVC, AND RVT). Their self-organizing map portraits show specific spot patterns, which were grouped into 7 cell clusters, C1–C7. The composition-flow presentation shows how cells in the different treatment groups are composed of the cell-type clusters. C1 and C6/C7 are specific for sensitive (S) and double-agent-resistant (RVC and RVT) cells, respectively, while C2–C5 correspond to cell subtypes present under all conditions but at varying levels of enrichment. Maximum diversity of cellular states is observed after single treatment (RV).