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. Author manuscript; available in PMC: 2022 May 9.
Published in final edited form as: Sci Signal. 2021 Nov 9;14(708):eabg2648. doi: 10.1126/scisignal.abg2648

Fig. 5. Selective KCC2 antagonist VU induced spontaneous epileptiform discharges in P7 pups.

Fig. 5.

(A to E) Representative EEG traces (A) and ictal event frequency raster plots (B) from P7 CD-1 pups that were administered either vehicle or VU0463271 (VU); black bars note i.p. injections. Expanded timescales show VU-induced epileptiform activity in the 1st- and 2nd-hour. 1st- vs. 2nd-hour ictal burden (C) and duration (D) after VU administration. Total frequency distribution for all interictal durations in P7 CD-1 pups administered VU(E). Vehicle, n=4 pups; VU, n=8 pups. (F to J) Representative EEG trace and seizure frequency raster plot (F) of P7 CD-1 pups that underwent unilateral carotid ligation with administration of VU 0.25 mg/kg at 1 hour (denoted by black bar). 1st- and 2nd-hour seizure burden (G) and duration (H). (I and J) 1st- and 2nd-hour seizure burden and duration plotted as percent PB-only. Data plots show all data points with means ± SEM. **P<0.05 and **P<0.01 by two-tailed paired t-test. Ligation + VU n=12 pups.