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. Author manuscript; available in PMC: 2022 May 9.
Published in final edited form as: Sci Signal. 2021 Nov 9;14(708):eabg2648. doi: 10.1126/scisignal.abg2648

Fig. 6. Effect of CLP290-mediated rescue of refractory seizures on post-ischemic KCC2 downregulation.

Fig. 6.

(A) Representative Western blots of KCC2 expression and Ser940 phosphorylation 24 hours after P7 ischemic seizures. N, naïve. (B) KCC2 and (C) Ser940 as percent ipsilateral/contralateral (right/left). (D) KCC2 and (E) Ser940 in left (“L”) and right (“R”) hemispheres. (F) Representative Western blots of KCC2 and Ser940 24 hours after P10 ischemic seizures. (G) KCC2 and (H) Ser940 as percent R/L. (I) KCC2 and (J) Ser940 in left and right hemispheres. (K) Representative western blot of KCC2 and Thr1007 24 hours after ischemic neonatal seizures. (L) Thr1007 phosphorylation as percent R/L. (M) Thr1007 phosphorylation in left and right hemispheres. *P<0.05, **P<0.01, and ***P<0.001 by one-way ANOVA vs. naive. #P<0.05, ##P<0.01 and ###P<0.001 vs. PB-Only. P7 pups: Naïve n=27, PB-only n=18, 5 Post n=3, 10 Post n=9, 20 Post n=13, 5’ n=4, 10’ n=11, 20 Pre n=9 pups. P10 pups: Naïve n=18, PB-only n=11, 10 Post n=6, 20 Post n=6, 10 Primed n=5, 20 Pre n=7 pups.