Fig. 3.

CAF40, Poseidon, and Zeus impact on viability. (A) Viability of RNAi-expressing flies, relative to the control genotype from each individual cross. The two hatched bars in the left show the relative viability of the two control GAL4-expressing drivers, Tub (ubiquitous) and T80 (imaginal-discs). CAF40-RNAi-expressing flies in red; Poseidon in blue and Zeus in orange. (B) Viability of homozygous flies for different CRISPR-Cas9 deletions for Poseidon (blue) and Zeus (orange) relative to the control from the same background strain (black). Notice the nonsignificant effect of the only nonframeshift deletion for Poseidon (first blue bar on the left); t-test: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate SD.