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. 2022 Jan 15;13:122–135. doi: 10.18632/oncotarget.28170

Figure 1. Effect of LA on the expression of genes encoding SHH pathway mediators in CT26 cells.

Figure 1

(A) Intracellular localization of ADAM-labeled LA. Mitochondria were labeled with MitoGreen. Right panel showed a single cell. Yellow circle indicates nucleus. Scale bar, 20 μm. (B) Gene expression of hedgehog ligand and receptors examined by RT-PCR. (C) Gene expression of hedgehog signaling pathway. (B, C) The graphs represent the results of quantitative RT-PCR. (D) Protein levels in nuclear fraction of hedgehog signaling pathway. The graphs represent semi-quantification of the results shown on the left. (A-D) CT26 cells were treated with 35 μM of LA, which was equivalent to inhibitory concentration 25. Error bars indicate standard deviations based on three independent experiments. Asterisks indicate statistical significance. Statistical differences were calculated by ordinary analysis of variance. Abbreviations: ADAM: 9-anthryldiazomethane; LA: linoleic acid; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; SHH: sonic hedgehog; PTCH: patched; SMO: smoothened; GLI: glioma-associated oncogene homolog.