Figure 2. Effect of LA on stemness in CT26 cells.

(A) Expression of stem cell-associated genes. (B) Sphere formation assay. The bar graph shows the quantification of total sphere numbers of 3 cell lines. Scale bar, 200 μm. (A, B) Cells were treated with 35 μM of LA, which was equivalent to inhibitory concentration 25. Error bars indicate standard deviations based on three independent experiments. Asterisks indicate statistical significance. Statistical differences were calculated by ordinary analysis of variance. Abbreviations: LA: linoleic acid; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; SOX: SRY-box transcription factor; PAX: paired box; NANOG: nanog homeobox; COL: collagen; OCT: octamer-binding transcription factor; NS: nucleostemin.