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. 2022 Jan 15;13:122–135. doi: 10.18632/oncotarget.28170

Figure 3. Effect of LA on generation of cleaved GLI2 in CT26 cells.

Figure 3

(A) Nuclear protein levels of GLI2 and cleaved GLI2 in CT26 cells. (B) Nuclear protein levels of determined using an anti-GLI2 N-terminus antibody. GAPDH was examined as a cytosolic marker. (C) Nuclear protein levels of GLI2 and cleaved GLI2 in Colo320 and HT29 cells (D) Effect of siGLI2 on nuclear GLI2 protein levels in LA-treated CT26 cells. (E) Effect of Gli2 knockdown on SOX17 expression in CT26 cells treated with LA. (F) Sphere formation assay. The bar graph shows the quantification of total sphere numbers of 3 cell lines. Production of SOX17 in untreated control, control siRNA-treated, and siGLI2-treated CRC cells. CRC cells were treated with 35 μM of LA, which was equivalent to inhibitory concentration 25. Abbreviations: LA: linoleic acid; GLI: glioma-associated oncogene homolog; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; siGLI2: short interference RNA for GLI2; siC: short interference RNA; SOX: SRY-box transcription factor; Cont: control.