Table 1. Primer pairs used in this study.
| Gene symbol | Gene bank ID | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
| Gapdh | NM_001289726.1 | aactttggcattgtggaagg | acacattgggggtaggaaca |
| Shh | NM_009170.3 | ctggccagatgttttctggt | gatgtcggggttgtaattgg |
| Ptch | NM_008957.3 | ctcaggcaatacgaagcaca | gacaaggagccagagtccag |
| Smo | NM_176996.4 | ttgtgctcatcaccttcagc | tgccaaacatggcaaataga |
| Gli1 | AB025922.1 | tctgtgatgggcaatggtct | tctggggtgggatcaggata |
| Gli2 | NM_001081125.1 | cagccttcacttttccccac | ctgcttgttctggttggcat |
| Gli3 | NM_008130.2 | tgcccatcagctactcagtg | ttgttgcagagtgaggttgc |
| Sox17 | BC060612.1 | gcacagcagaacccagatct | ccggtacttgtagttggggt |
| Pax6 | AF443223.1 | aggaaccagagaagacaggc | caggttgtttgccatggtga |
| Nanog | AY278951.1 | atgcggactgtgttctctca | tgctgagcccttctgaatca |
| Col1a | NM_007742.4 | atgtgccactctgactggaa | tccatcggtcatgctctctc |