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. 2022 Jan 3;12:794680. doi: 10.3389/fphar.2021.794680

FIGURE 5.

FIGURE 5

Quantification of γ-Glu-Glu in the extracellular medium of astroglioma C6 cells. (A) [GSH]i content in the control, BSO-, ACV-, and SFN- pretreated C6 cells. GSH was measured in cellulo with monobromobimane (mBrB) 24 h following the treatments. On the graph, data are presented as percentages of mBrB fluorescence intensity normalized to basal mBrB fluorescence measured in the control-untreated cells. ***p < 0.001 when comparing mBrB fluorescence measured in the control cells with either treatment (one-way ANOVA followed by the Holm–Sidak t-test). (B) Measurement of [γ-Glu-Glu] in the extracellular medium of C6 cells. Analysis of the C6 extracellular medium was performed on 100 µl samples of the culture medium of cells treated with BSO, ACV, and SFN. On the graph, [γ-Glu-Glu]e has been normalized to [γ-Glu-Glu]e detected in the control-untreated cells. **p < 0.01 and “not significant” (ns) when comparing [γ-Glu-Glu]e measured in control cells with either treatment (one-way ANOVA followed by the Holm–Sidak t-test).