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. Author manuscript; available in PMC: 2022 Jan 17.
Published in final edited form as: ACS Chem Biol. 2018 Aug 13;13(9):2568–2576. doi: 10.1021/acschembio.8b00513

Figure 2.

Figure 2.

Naïve fibronectin selection against α211. (a) mRNA display selection for the ecto-domain of α211. Binding over background was apparent at pool 3 and reached an appreciable level by pool 6. (b) Radioactive pull down of selected pool 6 fibronectin clones against 100 pmol of α211. We dubbed the clone with the highest level of binding α1-FANG1. (c) Competition of α1-FANG1 with α-Btx. 35S-labeled α1-FANG1 binds beads with immobilized α211; preincubation of α-Btx with α211-immobilized beads causes a decrease in the number of detectable counts of radiolabeled α1-FANG1. (d) α1-FANG1 performance under temperature stress. Pull down of radiolabeled α1-FANG1 against α211 shows α1-FANG1 inactivation at high temperatures.