Skip to main content
. Author manuscript; available in PMC: 2022 Jan 17.
Published in final edited form as: ACS Chem Biol. 2018 Aug 13;13(9):2568–2576. doi: 10.1021/acschembio.8b00513

Figure 3.

Figure 3.

Affinity maturation and stabilization of α1-FANG1. (a) Rounds 1–5 of the maturation selection show increased resistance to introduced pressures as the rounds proceed. Rounds 3–5 were exposed to heat in the form of a 65 °C incubation. Rounds 4 and 5 were exposed to Proteinase K (Pro-K). Round 5 included an off-rate competition with α-Btx. (b) Sequence information for clones derived via maturation selection, with α1-FANG2 highlighted. (c) Behavior of individual clones from α1-FANG1-based maturation selection. Individual clones show a range of resistance to the various selective pressures. (d) Comparative pull downs against α211 and the ecto-domain of rat α9 show that α1-FANG2 is highly selective for α211 over α9.