Figure 7.

α1-FANG3 protects against α-Btx inhibition of Ach-induced current in the Xenopus laevis oocyte expression system. (a) Preincubation of oocytes (n = 8) with 200 nM α1-FANG3 for 3 min prior to testing with 10 μM ACh reduces the ACh-induced current by ~5%. Preincubation of the same oocytes with 50 nM α-Btx for 3 min prior to additional testing with 10 μM ACh reduces the ACh-induced current by ~90%. (b) Preincubation of oocytes with 200 nM α1-FANG3 for 5 min and then a mixture of 200 nM α1-FANG3 and 50 nM α-Btx for 3 min shows a protection of ACh-induced current compared to incubations with α-Btx alone. Error bars represent the standard error of the mean (SEM). *** = p value ≤ 0.01