Table 2. Descriptions of antibody-related plasmids generated in this study.
Plasmids will be made available by contacting the corresponding author. Use of the plasmids and sequence information is for non-commercial purposes only.
| Plasmid name | Description |
|---|---|
| pDL001_Hec1-ms_IgG_HC | Hec1 heavy chain variable and constant regions (mouse) + exogenous N-term signal peptide |
| pDL002_Hec1-ms_IgG_LC | Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide |
| pDL003_Hec1-rb_IgG_HC | Hec1 heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide |
| pDL004_Hec1-rb_IgG_LC | Hec1 light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide |
| pDL005_Hec1-hu_IgG_HC | Hec1 heavy chain variable region + human heavy chain constant regions (UniProt P01857) + exogenous N-term signal peptide |
| pDL006_Hec1-hu_IgG_LC | Hec1 light chain variable region + human light chain constant region (UniProt P01834) + exogenous N-term signal peptide |
| pDL007_pMELT-rb_IgG_HC | KNL1 pMELT heavy chain variable and constant regions (rabbit) + exogenous N-term signal peptide |
| pDL008_pMELT-rb_IgG_LC | KNL1 pMELT light chain variable and constant region (rabbit) + exogenous N-term signal peptide |
| pDL009_CENPC-ms_IgG_HC | CENP-C heavy chain variable region + mouse heavy chain constant regions (from Hec1 sequence) + exogenous N-term signal peptide |
| pDL010_CENPC-ms_IgG_LC | CENP-C light chain variable region + mouse light chain constant region (from Hec1 sequence) + exogenous N-term signal peptide |
| pDL011_CENPC-hu_IgG_HC | CENP-C heavy chain variable region + human heavy chain constant regions (UniProt P01857) + exogenous N-term signal peptide |
| pDL012_CENPC-hu_IgG_LC | CENP-C light chain variable region + human light chain constant region (UniProt P01834) + exogenous N-term signal peptide |
| pDL013_BubR1-ms_IgG_HC | BubR1 heavy chain variable and constant regions (mouse); (contains endogenous N-term signal peptide) |
| pDL014_BubR1-ms_IgG_LC | BubR1 light chain variable and constant region (mouse); (contains endogenous N-term signal peptide) |
| pDL015_BubR1-hu_IgG_HC | BubR1 heavy chain variable region + human heavy chain constant regions (UniProt P01857) (contains endogenous N-term signal peptide) |
| pDL016_BubR1-hu_IgG_LC | BubR1 light chain variable region + human light chain constant region (UniProt P01834) (contains endogenous N-term signal peptide) |
| pDL017_Mad2C-ms_IgG_HC | Mad2-C heavy chain variable and constant regions (mouse) (contains endogenous N-term signal peptide) |
| pDL018_Mad2C-ms_IgG_LC | Mad2-C light chain variable and constant region (mouse) (contains endogenous N-term signal peptide) |
| pDL019_Mad2C-hu_IgG_HC | Mad2-C heavy chain variable region + human heavy chain constant regions (UniProt P01857) (contains endogenous N-term signal peptide) |
| pDL020_Mad2C-hu_IgG_LC | Mad2-C light chain variable region + human light chain constant region (UniProt P01834) (contains endogenous N-term signal peptide) |
| pDL023_Tub-rb_IgG_HC | α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide |
| pDL024_Tub-rb_IgG_LC | α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide |
| pDL025_HA-rb_IgG_HC_15F11 | HA-tag heavy chain hypervariable regions grafted into framework 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide |
| pDL026_HA-rb_IgG_LC_15F11 | HA-tag light chain hypervariable regions grafted into framework 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide |
| pDL027_scFvC_Hec1-rb | Hec1 heavy and light chain variable regions connected by linker + rabbit heavy chain constant regions 2 and 3 + exogenous N-term signal peptide |
| pDL028_scFvC_Mad2C-rb | Mad2-C heavy and light chain variable regions connected by linker + rabbit heavy chain constant regions 2 and 3 + exogenous N-term signal peptide |
| pDL029_scFv_pMELT_GFP_NoSP | KNL1 pMELT heavy and light chain variable regions connected by linker + GFP (no signal peptide included) |
| pDL030_scFvC_Hec1-rb_NoSP | Hec1 heavy and light chain variable regions connected by linker + rabbit heavy chain constant regions 2 and 3 (no signal peptide included) |
| pDL031_scFvC_Mad2C-rb_NoSP | Mad2-C heavy and light chain variable regions connected by linker + rabbit heavy chain constant regions 2 and 3 (no signal peptide included) |