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. 2021 Dec 23;10:e60311. doi: 10.7554/eLife.60311

Figure 1. 8 C administration attenuates ischemia-reperfusion injury in rats.

(A) Metabolic pathways of acetyl-CoA synthesis. (B) Schematic diagram of different carbon sources administration prior to I/R surgery. (C) Quantification of IS/AAR ratio in Figure 1—figure supplement 1A by Image J. (D) Schematic diagram of 8C administration post ischemic injury. (E) Representative figures of heart sections at 24 hr after I/R with or without 8C administration at reperfusion after 45 min ischemia. Scale bar: 2.5 mm (F) Quantification of IS/AAR ratio in (E). LV EF (G) and FS (H) at 24 hr after I/R. (I) Quantification of Acetyl-CoA levels in sham and I/R rat hearts at indicated conditions. (J–K) Western blot and quantification of H3K9ac and H3 in rat hearts 24 hr after I/R. (L) Trichrome masson staining of heart section after 4 weeks of I/R. Scale bar: 2.5 mm. (M) Quantification of infarct size in (I). LV EF (N) and FS (O) at 4 weeks after I/R. Error bars represent S.D. n = 5, #p < 0.05, ##p < 0.01 vs Sham group. * p < 0.05, **p < 0.01, vs I/R group. Data were analyzed by one-way ANOVA (C,G,H,K,N and O) or two-way ANOVA (I), followed by post-hoc Tukey test, data in F and M were analyzed by two-tailed student test.

Figure 1—source data 1. Original numeric data for Figure 1.
Figure 1—source data 2. Original western blot figure for Figure 1.

Figure 1.

Figure 1—figure supplement 1. Effect of metabolites on myocardial I/R injury.

Figure 1—figure supplement 1.

(A) Representative figures of heart sections at 24 hr after I/R in presence of different metabolites. Scale bar: 2.5 mm. (B) Quantification of AAR/LV in Figure 1A by Image J. (C) Quantification of AAR/LV ratio after I/R with or without 8C administration at 45 min after ligation. (D–E) Representative echo image for measurement of heart function in Figure 1G, H, N and O. (F) Quantification of Succinate levels in rat hearts after I/R with saline, 8C or 9C treatment. n = 3, *p < 0.05, **p < 0.01 vs I/R + saline group; ##p < 0.01 vs I/R + 8C group. Data were analyzed by one-way ANOVA (B and E), followed by post-hoc Tukey test, data in C was analyzed by two-tailed student test.