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. 2022 Jan 10;2022:2485250. doi: 10.1155/2022/2485250

Figure 4.

Figure 4

STAT6 positively regulates autophagy through VDR. (a) The mRNA levels of STAT6, Arg1, VDR, CYP24A, ATG7, Beclin1, and LC3B in lung tissue were measured by qRT-PCR assay. Results were expressed as means ± SD (n = 6, ∗P < 0.05, Stat6+/+ vs. Stat6−/−). (b) The protein expressions of STAT6, VDR, CYP24A, ATG7, and Beclin1 in BEAS-2B cells following different doses of siSTAT6 were measured using western blot analysis (n = 4; ∗P < 0.05, Ctrl vs. treatment groups). (c) The protein expressions of STAT6, p-STAT6, VDR, CYP24A, ATG7, and Beclin1 in BEAS-2B cells following different doses of IL4 were measured using western blot analysis (n = 4; ∗P < 0.05, Ctrl vs. treatment groups). (d) Cells were transfected with siVDR for 24 h and followed by IL4 (20 ng/ml, 24 h) treatment. The protein expressions of STAT6, p-STAT6, VDR, CYP24A, ATG7, and Beclin1 were detected by western blot analysis (n = 4; ∗P < 0.05, Ctrl vs. IL4; #P < 0.05, siCtrl vs. siVDR). (e) Immunofluorescence staining of LC3B in the cells with IL4 treatment. Nuclei were stained with DAPI (blue).