Table 3.

Interference factors and reagent amounts optimized for the complement-induced erythrocyte hemolysis test, detected by flow cytometry.

	Test tube 1	Test tube 2	Test tube 3	Test tube 4	Test tube 5
Serum (μL)	50	50	50	50	50
4 U hemolysin (μL)	100	100	100	0	0
4.4 U hemolysin (μL)	0	0	0	100	0
3.6 U hemolysin (μL)	0	0	0	0	100
2% erythrocytes (μL)	50	0	0	50	50
2.2% erythrocytes (μL)	0	50	0	0	0
1.8% erythrocytes (μL)	0	0	50	0	0