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Experimental system for flow cytometry-based detection of TCA.
| Control reaction | Testing reaction | |
|---|---|---|
| Buffer (μL) | 50 | 0 |
| Hemolysin (μL) | 100 | 100 |
| Serum (μL) | 0 | 50 |
| 2% human O-erythrocytes (μL) | 50 | 50 |
Note: when the same batch of 2% human O-erythrocytes and hemolysin as the reaction reagent was used, only the control reaction needed to be repeated five times to obtain the average control and agglutination coefficient of the five experiments for all serum samples tested in that batch.
