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K M and Vmax values for soluble and immobilized enzymes. The kinetic parameters were determined at each optimum temperature and the starch concentrations from 2.0 to 10.0 mg/mL. The values were shown as mean ± SD, n = 2.
| Sample | V max (μmole mL−1 min−1) | K M (mg mL−1 substrate) |
|---|---|---|
| Soluble enzyme | 10.90 ± 1.894 | 3.04 ± 1.043 |
| Immobilized enzyme | 1.44 ± 0.299 | 8.31 ± 2.666 |
