Table 1.

ICC, design effect, and effective sample size for the five groups in Example 1. ICC and the design effect were the lowest at 48h, when the data were relatively homogeneous across animals. At baseline and 72h, the data are noticeably heterogeneous across animals, leading to high ICC.

	Saline (7 mice)	24h (6 mice)	48h (3 mice)	72h (3 mice)	1wk (5 mice)
# of cells	357	209	139	150	245
ICC	0.61	0.33	0.02	0.63	0.54
Design effect	32.0	17.7	1.8	31.8	26.8
Effective sample size	11.1	17.5	76.9	4.7	9.1