Fig. 3.

Comparison of maximal developed force (F_max; A and G), resting tension (B and H), time-to-peak force (T_PF; C and I), time-to-half relaxation (T_0.5R; D and J), average rate of contraction (Rate_Rise; E and K) and average rate of 50% relaxation (Rate_{Relax 50%}; F and L) of isometrically-contracting ventricular strips from 5 °C Alaska blackfish acclimated to normoxia or hypoxia. Experimental preparations exposed to normoxia (N), acute hypoxia (H) and subsequent reoxygenation (R) are presented in panels A–F. Time control preparations maintained in oxygenated saline are presented in panels G–L. Statistical analysis: 2-way repeated-measures ANOVA and Student-Newman-Keuls multiple comparison post hoc test. A dagger (†) indicates a main effect of acclimation condition and a difference (P < 0.05) between normoxia- and hypoxia-acclimated preparations. Dissimilar uppercase letters indicate a main effect of and differences (P < 0.05) among recording conditions (or times). In panels A and K, where an interaction was found between acclimation and recording conditions (or times), an asterisk (*) indicates a difference (P < 0.05) between acclimation groups within a recording condition, whereas dissimilar lowercase letters indicate differences (P < 0.05) among recording conditions (or times) within an acclimation group. Data are means ± SEM. In all instances, n = 3 strips from N = 3 fish per acclimation group.