Fig. 5.

Overexpressed ADAM9 rescues the impaired abilities of migration and invasion of U87 cells induced by USP39 depletion. (A) Analysis of ADAM9 expression in U87 cells transfected with siNC or siUSP39 plus ADAM9 overexpressed (HA‐ADAM9) plasmid or EV plasmid determined by western blotting. (B) Transwell migration assay of U87 cells. Migrating cells were observed under a light microscope. The average number of migratory cells was counted in triplicate. Scale bar: 100 μm. (C) Transwell invasion assay of U87 cells. The average number of migratory cells was counted in triplicate. Scale bar: 100 μm. All data are presented as means ± SD. Significance calculated using Student’s t‐test. *P < 0.05, **P < 0.01. Representative data are from three independent experiments.