Figure 3.

Expression level of TNFα in patients and in animals. Representative image (A) and relative quantification (B) of TNFα staining in kidney biopsies obtained from patients diagnosed with chronic kidney disease (CKD). Normal tissue available from a partial nephrectomy in patients diagnosed with renal cancer were stained as control. Scale bar = 100 μm. Relative quantification of mRNA levels of Tnfα in kidney of rats that underwent 5/6 nephrectomy to induce CKD (C) or unilateral with contralateral nephrectomy to induce AKI (D). Data were normalized for the expression level of β-actin and expressed as mean ± SD relative to sham values. All comparisons were performed by unpaired Student t-test. In scatter plot representation, each dot represents one individual sample.