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. 2022 Jan 17;12:850. doi: 10.1038/s41598-021-04442-x

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Irgm1 deficiency is associated with CD8⁺ T cell cytokine overproduction and increased apoptosis. (a-b) Splenocytes were isolated from WT and Irgm1^−/− mice, stained and analyzed by flow cytometry for CD8⁺IFNγ⁺ (a) and CD8⁺Granzyme B⁺ (b). (c) CD8⁺ T cells were isolated and activated for 48 h with plate bound anti-CD3 and anti-CD28 antibodies after which the media was collected and IFNγ and Granzyme B were measured by ELISA. (d) Activated CD8⁺ T cells were assayed for the presence of apoptotic cells, measuring 7-AAD and annexin V positive cells by flow cytometry. (a,b) Data pooled from two independent experiments (n = 6–7), (c, d) data representative of two independent experiments (n = 3–4); error bars represent ± SEM. Mann Whitney test or T-test with Welch’s correction was used to compare groups depending on the normality of the distribution as judged by the Shapiro–Wilk test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.