Fig. 3.

KA weaken the glycolytic ability of thyroid cancer cells. A ECAR is a standard and comprehensive method to assess the key parameters of glycolytic capacity. The Glycolytic Capacity of C643 and 8505C were significantly decrease after treated with KA compared with the control group. B After KA treatment for 24 h, the lactic acid production of thyroid cancer cell line C643 was significantly reduced, while 8505C and K1 cell lines were not. C 5 mM Lactic acid was added to the medium separately or simultaneously with 4 μM KA. 5 mM L-lactic acid could partially alleviate the antiproliferative activity of KA just only in C643 cell line. D The ATP levels experienced a rapid, extensive decrease in four thyroid cancer cell lines after treated with KA, while with the higher KA concentrations, accompanied by the more ATP deprivation. Data are presented as mean ± SD of values from three different measurands. Statistically significant differences are indicated: *p < 0.05; **p < 0.01; ***p < 0.001 for comparison with control