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. 2022 Jan 4;15:779080. doi: 10.3389/fnbeh.2021.779080

FIGURE 1.

FIGURE 1

Representation of the experimental design. Control (standard diet; laboratory chow Nuvilab® CR-1) or high-salt (HS) (laboratory chow with added salt 4% NaCl non-iodized) diets were provided for parents at 21 day-old. They received their respective diets for 120 days, until they reached adulthood. During the periods of mating, gestation, and lactation, the dams remained on the aforementioned diets. The preconception, gestation, and lactation periods were classified as “preweaning.” At 21 day-old, the offspring were weaned and received until adulthood the control or HS diets. The period from weaning to adulthood was classified as “postweaning.” Therefore, the offspring were subdivided into the groups: control-control (C-C), offspring of standard diet fed dams who received a standard diet after weaning; control-HS (C-HS), offspring of standard diet fed dams who received a HS diet after weaning; HS-C, offspring of HS diet fed dams who received a standard diet after weaning; and HS-HS, offspring of HS diet fed dams who received a HS diet after weaning.