FIGURE 3.

Single-cell RNA sequencing (ScRNA-seq) analysis of HCC patients. (A) t-SNE clustering of scRNA-seq colored by significant cell types. (B) t-SNE plot of expression for genes specifically upregulated in each of the clusters. (C) t-SNE clustering of scRNA-seq as in (A) but colored by patient type. Normal: nontumor liver, Tumor: primary tumor, MLN: metastatic lymph node, PVTT: portal vein tumor thrombus. (D) t-SNE plot of expression for nine PRGs that were significantly highly expressed in tumor-related cell clusters. (E) Statistics of the cell counts of the nine PRGs coexpressed with EPCAM in 2,795 cancer stem cells (CSCs). (F) Regulatory network analysis of the nine PRGs using Ingenuity Pathway Analysis (IPA).