Table 1.

Dwell times for substrate interactions with yeast APC/C.

	Cdh1WD40	APC/CCdh1	APC/CCdc20	APC/CApc10-4A, Cdh1	APC/CApc10-4A, Cdc20
Hsl1 D box	0.360 s	35.3 s	0.412 s	0.375 s	0.152 s
Hsl1 D box mutant	No binding	No binding	No binding	No binding	No binding
ySecurin D box	SF	0.132 s	1.87 s	SF	SF
Clb5 D box	SF	0.159 s	0.207 s
Hsl1 KEN	0.166 s	0.185 s	No binding
ySecurin KEN	SF	SF	No binding
Acm1 ABBA	SF	No binding	No binding
Hsl1Halo KEN & D	56.4 s	321 s	0.518 s	75.9 s	0.449 s
Hsl1Halo KEN & ΔD	0.421 s	0.374 s	No binding
Hsl1Halo ΔKEN & D	3.10 s	41.6 s	SF	3.31 s	SF
Hsl1Halo ΔKEN & ΔD	No binding	No binding	No binding	No binding	No binding
AurKB D box	SF	No binding	No binding
hSecurin KEN	No binding	No binding	No binding

Dwell times are representative of two independent replicates, listed in Supplementary Table 1.

SF single frame.