Table 2.

Dwell times for substrate interactions with human activators.

	hCdh1WD40	hCdc20WD40
Hsl1 D box	4.52 s	1.08 s
Hsl1 D box mutant	No binding	No binding
ySecurin D box	SF	SF
Clb5 D box	SF	0.054 sa
Hsl1 KEN	1.48 s	SF
ySecurin KEN	0.869 s	SF
Acm1 ABBA	No binding	No binding
AurKB D box	0.202 s	SF
hSecurin KEN	0.174 s	No binding

Dwell times are representative of two independent replicates, listed in Supplementary Table 1.

SF single frame.

^aCalculated dwell time is less than three times the frame rate and is, therefore, less reliable.