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. 2022 Jan 17;12:827. doi: 10.1038/s41598-022-04804-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of intracellular metabolites at day 1 (myoblasts) and day 7 (myotubes) in DMEM, Serum, B27, and AIM-V cultures. (A) Heat map of the average order of magnitude of intracellular concentrations (M). D, S, B, and A indicate DMEM, Serum, B27, and AIM-V, respectively. (B) Contribution (%) of metabolite classes to the total measured levels of metabolites. (C) Score plot of Principal component analysis (PCA). (D) Hierarchical clustering analysis (HCA) based on average distance and Ward clustering algorithm was performed on Metaboanalyst. A1, B1, D1, and S1 indicate AIM-V, B27, DMEM, and Serum culture at day 1 (myoblasts), and A7, B7, D7, and S7 indicate AIM-V, B27, DMEM, and Serum culture at day 7 (myotubes). (n) = 4.