Figure 7.

(A) Simplified schematic alteration of central carbon metabolism in C2C12 myotubes versus myoblasts with a heat map of the log2 fold change of average metabolites levels in Serum, B27, and AIM-V cultures. The student’s t-test was performed. Bold lines indicate statistically significant metabolite levels (p < 0.05). Dark gray color metabolites were not determined. Direct reactions are shown by continuous lines, a series of reactions are indicated by dashed lines. TCA tricarboxylic acid, PPP pentose phosphate pathway, Glc ex consumption of glucose, Gln ex consumption of glutamine, Lac ex secretion of lactate. Significant different pyruvate metabolisms were shown in myotubes cultured in Serum, B27, AIM-V at day 7. The graphs were illustrated using OMIX 2.0 software⁵⁹. (B) Pyruvate reduction into Lactate and (C) Transamination to Alanine. (n) = 4. **p < 0.01. Data presented as average ± standard deviation (SD).