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. 2022 Jan 17;5:61. doi: 10.1038/s42003-021-02989-z

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Fig. 1 — Fluorescence polarisation anisotropy (FPA) to determine RNA binding affinities of recombinant 3D^pol-WT and 3D^pol-GNN. a Binding of FITC-labelled 13mer poly-A RNA to recombinant 3D^pol calculated as average anisotropy after 30 min incubation. b Mean Kd values comparing the affinity of 3D^pol-WT and 3D^pol-GNN with 13mer FITC-labelled poly-A RNA (n = 3 ± SEM, analysed by interpolation of a standard curve, two-tailed unpaired t-test, **p < 0.01).