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. 2022 Jan 17;5:61. doi: 10.1038/s42003-021-02989-z

Fig. 2. Polymerase activity assay.

Fig. 2

Polymerase activity assays were used to determine activity of recombinant 3Dpol. a Dot-blot showing that incorporation of [α-32P] UTP is only possible when all assay components are included. b Quantification of levels of incorporated radiolabelled nucleotides after 30 min incubation by scintillation counts per minute normalised to WT (n = 3 ± SEM, analysed by two-tailed unpaired t test, ****p < 0.0001). The GNN mutant indicates background levels.