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. 2022 Jan 17;12:795. doi: 10.1038/s41598-022-04856-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Age-dependent reduction of PDGFB expression and effect of laminin-511. mRNA expression levels of PDGFB in cultured keratinocytes from donors of different ages were analyzed by qPCR (A). Data are expressed as mean ± SD of three independent experiments from 6 donors each in the 10–20’s and 50–60’s. * p < 0.05. mRNA expression levels of PDGFB in epidermis of the skin equivalent model treated with or without MMP inhibitor CGS27023A and heparinase inhibitor BIPBIPU were analyzed by qPCR (B). Data are expressed as mean ± SD of three independent experiments. ** p < 0.01. Protein membrane array analysis was performed by using cell lysate from keratinocytes cultured on laminin-511 E8 fragment-coated or non-coated plates (C). mRNA expression levels of PDGFB in cultured keratinocytes from donors of different ages cultured on laminin-511 E8 fragment-coated or non-coated plates (D) were analyzed by qPCR. Data are expressed as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01.